Vol. 23 No. 3 (2020) Cover Image
Vol. 23 No. 3 (2020)

Published: November 30, 2020

Pages: 225-231

Articles

Bio-Cementation of Sandy Soil through Bacterial Processing to Precipitate Carbonate

Abstract

Bio-cement built on microbial induced carbonate precipitation MICP, be able to consolidate the loose grains and can applied for soil reinforcement. In this study, the performing of an ureolytic Sporosarcina Pasteurii for sand stabilization was estimated. The S. Pasteurii Could effectively consolidates sand particles through urea hydrolysis and the successive production of calcite. The bio improved sands had relative great compressive strength after 60 days exposure to bacterial cells injections cycles. The compressive strength of bio stabilized sands was reliant on the utilized cell concentrations and density of urea and CaCl2. High bacteria cell masses decreased the compressive strength. The optimal density of cell, was OD600 0.5, when cost and performance were taken into account. The study shows that bio cementation of sand built on microbial induced carbonate precipitation (MICP) has ability for the reduction of sand permeability through pore clogging with precipitated carbonate.

References

  1. Fujita, Y., J. Taylor, L. Wendt, D. Reed, and R. Smith (2010), Evaluating the potential of native ureolytic microbes to remediate a (90)sr contaminated environment, Environ. Sci. Technol., 44(19), 7652–7658.
  2. DeJong, J. T. (2006), Microbially induced cementation to control sand response to undrained shear, J. Geotech. Geoenviron. Eng., 132(11), 1381.
  3. van Paassen, L. A., R. Ghose, T. J. M. van der Linden, W. R. L. van der Star, and M. C. M. van Loosdrecht (2010), Quantifying biomediated ground improvement by ureolysis: Large-scale biogrout experiment, J. Geotech. Geoenviron. Eng., 136(12), 1721–1728.
  4. Whiffin, V. S., L. A. van Paassen, and M. P. Harkes (2007), Microbial carbonate precipitation as a soil improvement technique, Geomicrobiol. J., 24(5), 417–423.
  5. Cunningham, A. B., R. Gerlach, L. Spangler, and A. C. Mitchell (2009), Microbially enhanced geologic containment of sequestered supercritical CO2, Energy Procedia, 1(1), 3245–3252.
  6. Ferris, F., V. Phoenix, Y. Fujita, and R. Smith (2003), Kinetics of calcite precipitation induced by ureolytic bacteria at 10 to 20 degrees C in artificial groundwater, Geochim. Cosmochim. Acta, 67(8), 1701–1710.
  7. Mobley, H. L. T., and R. P. Hausinger (1989), Microbial ureases-significance, regulation, and molecular characterization, Microbiol. Rev., 53(1), 85–108.
  8. Stumm, W., and J. J. Morgan (1996), Aquatic Chemistry: Chemical Equilibria and Rates in Natural Waters, 3rd ed., xvi, 1022 pp., Wiley, N. Y.
  9. Fujita, Y., J. L. Taylor, T. L. T. Gresham, M. E. Delwiche, F. S. Colwell, T. L. McLing, L. M. Petzke, and R. W. Smith (2008), Stimulation of microbial urea hydrolysis in groundwater to enhance calcite precipitation, Environ. Sci. Technol., 42(8), 3025–3032.
  10. Harkes, M. P., L. A. van Paassen, J. L. Booster, V. S. Whiffin, and M. C. M. van Loosdrecht (2010), Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement, Ecol. Eng., 36(2), 112–117.
  11. De Muynck, W., K. Verbeken, N. De Belie, and W. Verstraete (2010b), Influence of urea and calcium dosage on the effectiveness of bacterially induced carbonate precipitation on limestone, Ecol. Eng., 36(2), 99–111.
  12. Mohammed A., Al-Omarib R., Mustafa H., (2017), Experimental Study to Improve the Shear Stress of Silty- Sandy Soils by Using Urease Producing Bacteria, American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) ISSN (Print) 2313-4410, ISSN (Online) 2313-4402
  13. Achal, V., A. Mukherjee, P. C. Basu, and M. S. Reddy (2009b), Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production, J. Ind. Microbiol. Biotechnol., 36(7), 981–988.
  14. Tobler, D. J., M. O. Cuthbert, R. B. reswell, M. S. Riley, J. C. Renshaw, S. Handley-Sidhu, and V. R. Phoenix (2011), Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite, Geochim. Cosmochim. Acta, 75(11), 3290–3301, doi:10.1016/ j.gca.2011.03.023.
  15. DeJong, J. T., K. Soga, S. A. Banwart, W. R. Whalley, T. R. Ginn, D. C. Nelson, B. M. Mortensen, B. C. Martinez, and T. Barkouki (2012), Soil engineering in vivo: Harnessing natural biogeochemical systems for sustainable, multi-functional engineering solutions, J. R. Soc., Interface, 8(54), 1–15.
  16. Ramachandran SK, Ramakrishnan V, Bang SS (2001), Remediation of concrete using micro-organisms. ACI Mater J 98:3–9
  17. Ferris, F., L. Stehmeier, A. Kantzas, and F. Mourits (1996), Bacteriogenic mineral plugging, J. Can. Petrol. Technol., 35, 56–61.
  18. Inagaki, Y., Tsukamoto, M., Mori, H., Sasaki, T., Soga, K., Al. Qabany, A., Hata, T., 2011. The influence of injection conditions and soil types on soil improvement by microbial functions. ASCE, Geo-Frontiers.4021-4030.
  19. Mitchell JK and Santamarina JC (2005) Biological considerations in geotechnical engineering. Geotechnical and Geoenvironmental Engineering 131(10): 1222–1233.
  20. Rebata-Landa V (2007) Microbial Activity in Sediments: Effects on Soil Behavior. PhD thesis, Georgia Institute of Technology, Atlanta, GA, USA.
  21. Ciurli, S., C. Marzadori, S. Benini, S. Deiana, and C. Gessa (1996), Urease from the soil bacterium Bacillus pasteurii: Immobilization on Ca- polygalacturonate, Soil Biol. Biochem., 28(6), 811–817.
  22. O'Kelly Brendan, Rahim Shahrokhi, Seyed M. Zomorodian and Ali Niazi (2015) Improvement Sand with Microbial Induced Carbonate Precipitation, institute of cicil engineering, v168(3), 1755-0750.
  23. Sharma, S.S., and Fahey, M. (2003b). Evaluation of Cyclic Shear Strength of Two Cemented Calcareous Soils. Journal of Geotechnical and Geo environmental engineering 608-618.
  24. Shafii, R.-N., Clough, W., 1982. The Influence of Cementation on the Static and Dynamic Behavior of Sands. Stanford, California, California.